| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 401 | Brevibacillus brevis | TGTCAGCGCTGCATGGAA | ATCCCTTGCGCATCAGCA | 59.97 | 59.73 | 286 | 98 |
100.00%
|
100.00%
|
| Primer 402 | Brevibacillus brevis | AATGCGCTACACGGCTGT | AATGCTGCTCCCATCGCA | 60.05 | 59.73 | 177 | 98 |
100.00%
|
100.00%
|
| Primer 403 | Brevibacillus brevis | AAGACGGTTGGCAGCTGT | AATAGCTCGCTCCGCATCC | 59.49 | 60.01 | 283 | 98 |
100.00%
|
100.00%
|
| Primer 404 | Brevibacillus brevis | TGCCGTCCAGCAAGTGAT | TAGCCTTGCTTGCGTGCT | 59.25 | 59.97 | 288 | 98 |
100.00%
|
100.00%
|
| Primer 405 | Brevibacillus brevis | ATGCGATTCGTGCAGGCT | ACCGGAAGAGCCAGCTTTC | 60.12 | 60.00 | 242 | 98 |
100.00%
|
100.00%
|
| Primer 406 | Brevibacillus brevis | ATGCGATTCGTGCAGGCT | GAAACCGGAAGAGCCAGCT | 60.12 | 60.00 | 245 | 98 |
100.00%
|
100.00%
|
| Primer 407 | Brevibacillus brevis | ATGCGATTCGTGCAGGCT | TCCCCTTGACCTCCAACCA | 60.12 | 60.07 | 157 | 98 |
100.00%
|
100.00%
|
| Primer 408 | Brevibacillus brevis | ATGCGATTCGTGCAGGCT | AAACCGGAAGAGCCAGCT | 60.12 | 58.84 | 244 | 98 |
100.00%
|
100.00%
|
| Primer 409 | Brevibacillus brevis | TGAGGAGTGCGTCTTGCTG | TTTGGCTTGCATCGCGTC | 60.01 | 59.44 | 223 | 98 |
100.00%
|
100.00%
|
| Primer 410 | Brevibacillus brevis | AGCAGGCATTGACGCTGT | GCGCTCGACAAAAGCTGT | 59.97 | 59.06 | 198 | 98 |
100.00%
|
100.00%
|